Mammalian Cell Extraction Kit (ab65399)
Key features and details
- Assay type: Direct
- Assay time: 20 min
- Sample type: Adherent cells, Suspension cells, Tissue
製品の概要
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製品名
Mammalian Cell Extraction Kit -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Direct -
全工程の試験時間
0h 20m -
製品の概要
Abcam's Mammalian Cell Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g. caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K269 Mammalian Cell Extraction Kit. K269-500 is the same size as the 500 test size of ab65399.
製品の特性
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保存方法
Please refer to protocols. -
内容 500 tests Cell Extraction Buffer 1 x 100ml DTT 1 x 110µl Protease Inhibitor Cocktail 1 vial -
研究分野
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関連性
Mammalian Cell Extraction methods allow extraction of mammalian proteins from cultured cells and tissue samples.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab65399 は 19 報の論文で使用されています。
- Li H et al. The Src-Kinase Fyn is Required for Cocaine-Associated Memory Through Regulation of Tau. Front Pharmacol 13:769827 (2022). PubMed: 35185557
- Adema V et al. Targeting the EIF2AK1 Signaling Pathway Rescues Red Blood Cell Production in SF3B1-Mutant Myelodysplastic Syndromes With Ringed Sideroblasts. Blood Cancer Discov 3:554-567 (2022). PubMed: 35926182
- Li H et al. 4R Tau Modulates Cocaine-Associated Memory through Adult Dorsal Hippocampal Neurogenesis. J Neurosci 41:6753-6774 (2021). PubMed: 34099513
- Bottino LZMF et al. Extracellular vesicles derived from head and neck squamous cells carcinoma inhibit NLRP3 inflammasomes. Curr Res Immunol 2:175-183 (2021). PubMed: 35492395
- Román-Meléndez GD et al. Protease Activity Profiling via Programmable Phage Display of Comprehensive Proteome-Scale Peptide Libraries. Cell Syst 11:375-381.e4 (2020). PubMed: 33099407