製品の概要

  • 製品名Anti-Mammaglobin A antibody [31A5]
    Mammaglobin A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [31A5] to Mammaglobin A
  • 特異性Reacts with human mammaglobin A, no cross-reaction with lypophilin B and lypophilin C (mammaglobin B).
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
  • 免疫原

    Recombinant full length protein (Human) Mammaglobin A protein

  • ポジティブ・コントロール
    • Breast carcinoma, skin (eccrine sweat glands).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: Use at an assay dependent dilution.

    IHC-P: Use at a concentration of 1 µg/ml. Perform antigen retrieval step using Citrate buffer, or buffered EDTA, or Glycol based solution before commencing with IHC staining protocol.
    This antibody is suitable for the detection of occult mamma carcinoma cells in sentinel lymph nodes and in peripheral blood. The overall sensitivity of mammaglobin detection on paraffin sections of mamma carcinoma was reported to be >80% (Fanger et al. 2002; Han et al. 2003).

    WB: Use at an assay dependent dilution. See PubMed ID: 14521461

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-Mammaglobin A antibody [31A5] 画像

    • ab82203 at 1µg/ml staining Mammaglobin A in sentinel lymph nodes in metastasis of breast carcinoma.
    • ab82203 at 1µg/ml staining Mammaglobin A over-expression in breast carcinoma in situ.

    Anti-Mammaglobin A antibody [31A5] (ab82203) 使用論文

    This product has been referenced in:
    • Plante I  et al. Cx43 suppresses mammary tumor metastasis to the lung in a Cx43 mutant mouse model of human disease. Oncogene : (2010). IHC-P ; Mouse . Read more (PubMed: 21151177) »
    • Han JH  et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 127:1330-4 (2003). WB, IHC-P, ELISA ; Human, Mouse, Monkey . Read more (PubMed: 14521461) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (4T1 breast tumor metastases in lung and liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization Yes - Triton
    Specification 4T1 breast tumor metastases in lung and liver
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 27 2015

    Thank you for getting back to me and for confirming the order details. Currently, ab82203 is the only antibody against Mammaglobin A which has been tested for cross-reaction with mouse samples. Although, we have some other antibodies against the same t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"