製品の概要

  • 製品名Anti-MAML1 antibody
    MAML1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAML1
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
    • 組織特異性Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
    • 配列類似性Belongs to the mastermind family.
    • ドメインThe C-terminal region is required for transcriptional activation.
    • 細胞内局在Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    Anti-MAML1 antibody 画像

    • All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 108 kDa


      Exposure time : 30 seconds
    • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
      Chemiluminescence with exposure times of 30 seconds.

    Anti-MAML1 antibody (ab70486) 使用論文

    ab70486 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70486.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"