製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAML1
  • 特異性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
    • 組織特異性
      Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
    • 配列類似性
      Belongs to the mastermind family.
    • ドメイン
      The C-terminal region is required for transcriptional activation.
    • 細胞内局在
      Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    画像

    • All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 108 kDa


      Exposure time : 30 seconds
    • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
      Chemiluminescence with exposure times of 30 seconds.

    参考文献

    ab70486 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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