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Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus MAML1.
(Peptide available as ab66491.)
Our Abpromise guarantee covers the use of ab65090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
MAML1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAML1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65090.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 117kDa; MAML1
ab65090 has not yet been referenced specifically in any publications.