Anti-Maltose Binding Protein 抗体 [R29.6] (ab65)

製品の概要

  • 製品名
    Anti-Maltose Binding Protein antibody [R29.6]
    Maltose Binding Protein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [R29.6] to Maltose Binding Protein
  • アプリケーション
    適用あり: ICC, IP, WB, ChIPmore details
  • 免疫原

    MOS maltose binding protein fusion protein.

  • 特記事項
    MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    R29.6
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 細胞内局在
    Periplasm
  • 別名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

画像

  • Immunofluorescence analysis of Saos-2 cells, transfected with full-length MBP-Apoptin and MBP-Apoptin(80–121). After fixation, the cells were stained for Maltose Binding Protein using ab65. Nuclear morphology is indicated by DAPI staining.
  • Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.

参考文献

This product has been referenced in:
  • Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). Read more (PubMed: 21057026) »
  • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). Read more (PubMed: 19686684) »

See all 6 Publications for this product

レビューと Q&A

Unfortunately, the epitope recognized by product ab65 has not yet been mapped.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Saccharomyces cerevisiae Cell (S. cerevisae)
Specification
S. cerevisae
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Other
Positive control
negative control: MBP-expressing vector in yeast
Username

Mr. Xiao Liu

Verified customer

投稿 Aug 11 2005

This antibody was raised against a fusion protein and unfortunately we don't have the amino acid sequence.

I am afraid that this antibody has not been tested yet in ELISA. We also don't currently provide a conjugation service for our antibodies, however we do supply a range of conjugated secondary antibodies that may be suitable for your needs.

The optimal dilution should be determined for each application , but a good start point for Western blots would be 1µg/ml

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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