Anti-Maltose Binding Protein 抗体 [MBP-17] (HRP) (ab49923)

Abreviews (4) Submit a Question Specific References (5)

製品の概要

  • 製品名
    Anti-Maltose Binding Protein antibody [MBP-17] (HRP)
    Maltose Binding Protein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MBP-17] to Maltose Binding Protein (HRP)
  • 由来種
    Mouse
  • 標識
    HRP
  • 特異性
    This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • アプリケーション
    適用あり: WB, ELISA, Dot blotmore details
  • 免疫原

    Recombinant full length Maltose Binding Protein fusion protein

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab119994 as a replacement.

法規制情報

医薬用外毒物

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項（精製）
    The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • ポリ/モノ
    モノクローナル
  • クローン名
    MBP-17
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 細胞内局在
    Periplasm
  • 別名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

画像

  • ELISA - Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)
    ELISA - Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)This image is courtesy of an anonymous Abreview

    ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Christian F  et al. PKA phosphorylation of p62/SQSTM1 regulates PB1 domain interaction partner binding. Biochim Biophys Acta 1843:2765-74 (2014). Read more (PubMed: 25110345) »

See all 5 Publications for this product

ab49923 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
Votes: Highest First

ELISA abreview for Anti-Maltose Binding Protein antibody [MBP-17] (HRP)

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification
Recombinant Yeast SUMO protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Direct
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投稿 May 12 2011

Western blot abreview for Anti-Maltose Binding Protein antibody [MBP-17] (HRP)

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jul 22 2009

ELISA abreview for Anti-Maltose Binding Protein antibody [MBP-17] (HRP)

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Recombinant protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
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投稿 May 06 2009

Western blot abreview for Anti-Maltose Binding Protein antibody [MBP-17] (HRP)

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
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Verified customer

投稿 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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