Anti-Maltose Binding Protein 抗体 [MBP-17] (HRP) (ab49923)

製品の概要

  • 製品名Anti-Maltose Binding Protein antibody [MBP-17] (HRP)
    Maltose Binding Protein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MBP-17] to Maltose Binding Protein (HRP)
  • 標識HRP
  • 特異性This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • アプリケーション適用あり: WB, ELISA, Dot blotmore details
  • 免疫原

    Recombinant full length Maltose Binding Protein fusion protein

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • ポリ/モノモノクローナル
  • クローン名MBP-17
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 関連性Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • 細胞内局在Periplasm
  • 別名
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody [MBP-17] (HRP) 画像

  • ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923) 使用論文

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Beck J  et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013). Read more (PubMed: 23455478) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification Recombinant Yeast SUMO protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type Direct
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Verified customer

投稿 May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (BL21(DE3))
Loading amount 1 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

投稿 Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Recombinant protein (neuronal)
Specification neuronal
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type Indirect
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投稿 May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (E.Coli)
Loading amount 0.05 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

投稿 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"