Anti-Maltose Binding Protein 抗体 [MBP-17] (HRP) (ab49923)
製品の概要
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製品名Anti-Maltose Binding Protein antibody [MBP-17] (HRP)
Maltose Binding Protein 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [MBP-17] to Maltose Binding Protein (HRP)
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由来種Mouse
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標識HRP
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特異性This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
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アプリケーション適用あり: WB, ELISA, Dot blotmore details
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免疫原
Recombinant full length Maltose Binding Protein fusion protein
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特記事項
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab119994 as a replacement.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
精製度IgG fraction
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特記事項（精製）The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
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ポリ/モノモノクローナル
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クローン名MBP-17
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Related Products
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アプリケーション
Our Abpromise guarantee covers the use of ab49923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 43 kDa. | |
| ELISA | Use at an assay dependent dilution. | |
| Dot blot | Use at an assay dependent dilution. |
ターゲット情報
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関連性Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
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細胞内局在Periplasm
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別名
- ECK4026 antibody
- JW3994 antibody
- Mal E antibody
see all
画像
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![ELISA - Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)](http://a.abcam.com/ps/products/49/ab49923/Images/Maltose-Binding-Protein-Primary-antibodies-ab49923-3.jpg)
ELISA - Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)This image is courtesy of an anonymous Abreviewab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.
プロトコール
参考文献
This product has been referenced in:
- Pollard DJ et al. Broad-Spectrum Regulation of Nonreceptor Tyrosine Kinases by the Bacterial ADP-Ribosyltransferase EspJ. MBio 9:N/A (2018). Read more (PubMed: 29636436) »
- Ishida K et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"