製品の概要

  • 製品名Anti-MALT1 antibody [EP603Y]
    MALT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP603Y] to MALT1
  • 特異性This antibody is predicted to detect splice isoform 2 based on sequence analysis.
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MALT1 aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • WB: Ramos, HeLa and Jurkat cell lysates. ICC/IF: Ramos cells. Flow Cyt: Jurkat cells.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
Flow Cyt 1/100.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.
    • 組織特異性Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.
    • 関連疾患Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
    • 配列類似性Belongs to the peptidase C14B family.
      Contains 1 death domain.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • 細胞内局在Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Caspase like protein antibody
      • DKFZp434L132 antibody
      • MALT 1 antibody
      • MALT associated translocation antibody
      • MALT lymphoma associated translocation antibody
      • MALT lymphoma-associated translocation antibody
      • Malt1 antibody
      • MALT1_HUMAN antibody
      • MLT 1 antibody
      • MLT antibody
      • MLT1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation gene 1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Mucosa-associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Paracaspase antibody
      see all

    Anti-MALT1 antibody [EP603Y] 画像

    • Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling MALT1 with purified ab33921 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • All lanes : Anti-MALT1 antibody [EP603Y] (ab33921) at 1/10000 dilution (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 92 kDa
      Observed band size : 92 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-MALT1 antibody [EP603Y] (ab33921) at 1/2000 dilution (unpurified) + Jurkat cell lysate

      Predicted band size : 92 kDa
      Observed band size : 92 kDa
    • Flow Cytometry analysis of Jurkat cells labelling MALT1 with purified ab33921 at 1/100 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Overlay histogram showing Jurkat cells stained with unpurified ab33921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab33921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-MALT1 antibody [EP603Y] (ab33921) 使用論文

    This product has been referenced in:
    • Baens M  et al. MALT1 auto-proteolysis is essential for NF-?B-dependent gene transcription in activated lymphocytes. PLoS One 9:e103774 (2014). WB . Read more (PubMed: 25105596) »
    • Chan W  et al. A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival. Mol Cell Biol 33:429-43 (2013). WB ; Human . Read more (PubMed: 23149938) »

    See all 4 Publications for this product

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