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ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.
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ab112141 should be stored desiccated.
|Assay Buffer||1 x 25ml|
|DMSO||1 x 200µl|
|MalemGreen indicator||1 vial|
|N-ethylmaleimide Standard||1 x 50µl|
|Reaction Buffer||1 x 500µl|
Our Abpromise guarantee covers the use of ab112141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|