製品の概要

  • 製品名Anti-MAL antibody
    MAL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAL
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Frog
  • 免疫原

    Synthetic peptide corresponding to Human MAL aa 114-126.
    Sequence:

    CFDGFTY(R/K)HYHENI


    (Peptide available as ab39812)

  • ポジティブ・コントロール
    • Rat kidney extracts.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab15418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).Can be blocked with MAL peptide (ab39812).
ICC/IF Use at an assay dependent concentration. PubMed: 20861303
IP Use at an assay dependent concentration. PubMed: 20861303

ターゲット情報

Anti-MAL antibody 画像

  • All lanes : Anti-MAL antibody (ab15418) at 1/5000 dilution

    Lane 1 : mpkCCD cell lysate
    Lane 2 : Xenopus 2f3 cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit

    Predicted band size : 18 kDa
  • IHC image of ab15418 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab15418, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MAL antibody (ab15418) 使用論文

This product has been referenced in:
  • Carmosino M  et al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell 21:3985-97 (2010). ICC/IF, IP ; Rat, Dog . Read more (PubMed: 20861303) »
  • Kamsteeg EJ  et al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A 104:16696-701 (2007). IP ; Rat . Read more (PubMed: 17940053) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Schwann Cells)
Loading amount 100 µg
Specification Schwann Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jan 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"