製品の概要

  • 製品名Anti-MAK (phospho Y159) antibody
    MAK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAK (phospho Y159)
  • 特異性ab92562 detects endogenous levels of MAK only when phosphorylated at tyrosine 159.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human MAK around the phosphorylation site of tyrosine 159 (TDYPVS).

  • ポジティブ・コントロール
    • HepG2 cells (treated with PMA at 125ng/ml for 30mins)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab92562 was purified by affinity-chromatography using an epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 71 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
    • 組織特異性Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
    • 関連疾患Defects in MAK are the cause of retinitis pigmentosa type 62 (RP62) [MIM:614181]. RP62 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated. Phosphorylated on serine and threonine residues.
    • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Midbody. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Localized in both the connecting cilia and the outer segment axonemes (By similarity). Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • intestinal cell kinase antibody
      • dJ417M14.2 antibody
      • fj04c02 antibody
      • Ick antibody
      • Mak antibody
      • MAK_HUMAN antibody
      • Male germ cell associated kinase antibody
      • Male germ cell-associated kinase antibody
      • male germ cell-associated kinase retinal-enriched isoform antibody
      • OTTHUMP00000016025 antibody
      • Rck antibody
      • RP62 antibody
      • Serine/threonine protein kinase MAK antibody
      • Serine/threonine-protein kinase MAK antibody
      • testicular secretory protein Li 28 antibody
      • wu:fj04c02 antibody
      • zgc:56603 antibody
      see all

    Anti-MAK (phospho Y159) antibody 画像

    • All lanes : Anti-MAK (phospho Y159) antibody (ab92562) at 1/500 dilution

      Lane 1 : HepG2 cells, treated with PMA (125ng/ml, 30mins)
      Lane 2 : HepG2 cells, treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 71 kDa

    Anti-MAK (phospho Y159) antibody (ab92562) 使用論文

    ab92562 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92562.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"