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Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (?max = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
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Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
|Magnesium Assay Buffer||WM||1 x 25ml|
|Magnesium Developer||Purple||1 unit|
|Magnesium Enzyme Mix||Green||1 unit|
|Magnesium Standard||Yellow||1 x 0.1ml|
Our Abpromise guarantee covers the use of ab102506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample
Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted
ab102506 has not yet been referenced specifically in any publications.