製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAGI1
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C terminus of last 50 aa of Human BAIAP1, based on the mutated sequence in Q96QZ7-3 or 4.

  • ポジティブ・コントロール
    • Mouse brain cell lysate and Human hepatocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.
  • 組織特異性
    Widely expressed with the exception of skeletal muscle. Isoform 1, isoform 2 and isoform 6 are highly expressed in colon, kidney, lung, liver, and pancreas. Isoform 5 is predominantly expressed in brain and heart. Isoform 3 and isoform 4 are highly expressed in pancreas and brain.
  • 配列類似性
    Contains 1 guanylate kinase-like domain.
    Contains 6 PDZ (DHR) domains.
    Contains 2 WW domains.
  • 細胞内局在
    Cell junction, tight junction. Cell membrane. Localizes to epithelial cells tight junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIP 3 antibody
    • AIP-3 antibody
    • AIP3 antibody
    • Atrophin 1 interacting protein 3 antibody
    • Atrophin-1-interacting protein 3 antibody
    • BAI1 associated protein 1 antibody
    • BAI1-associated protein 1 antibody
    • BAIAP 1 antibody
    • BAIAP1 antibody
    • BAP 1 antibody
    • BAP-1 antibody
    • BAP1 antibody
    • Gukmi1 antibody
    • MAGI 1 antibody
    • MAGI-1 antibody
    • MAGI1 antibody
    • MAGI1_HUMAN antibody
    • MAGI1c antibody
    • Membrane associated guanylate kinase inverted 1 antibody
    • membrane associated guanylate kinase WW and PDZ domain containing 1 antibody
    • Membrane-associated guanylate kinase antibody
    • Membrane-associated guanylate kinase inverted 1 antibody
    • TNRC 19 antibody
    • TNRC19 antibody
    • Trinucleotide repeat containing gene 19 antibody
    • Trinucleotide repeat containing gene 19 protein antibody
    • Trinucleotide repeat-containing gene 19 protein antibody
    • WW and PDZ domain-containing protein 1 antibody
    • WW domain containing protein 3 antibody
    • WW domain-containing protein 3 antibody
    • WWP 3 antibody
    • WWP3 antibody
    see all

画像

  • Anti-MAGI1 antibody (ab37543) at 1/100 dilution + Mouse brain cell lysate

    Predicted band size : 137 kDa
    Observed band size : 137 kDa
    Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab37543, at a 1/50 dilution, staining human hepatocarcinoma tissue by immunohistochemistry, formalin-fixed paraffin-embedded tissue, followed by AEC staining.

参考文献

This product has been referenced in:
  • Ridgway LD  et al. MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface. Am J Physiol Cell Physiol 297:C55-65 (2009). Read more (PubMed: 19403801) »

See 1 Publication for this product

レビューと Q&A

Thank you for your patience. Here is the clarifying explanation regarding the immunogen: The immunogenic sequence was chosen based on the mutated sequence in Q96QZ7-3 or 4 and therefore it is absent in other isoforms including isoform 1.  It is i...

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Thank you for contacting us. I heard back from the lab with the following information: ab37543 can potentially recognize isoform 3 or 4. However, I am confused about this reply since the isoforms 3 and 4 (according to the UniProt database entry...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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