製品の概要

  • 製品名Anti-MAGED2 antibody
    MAGED2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAGED2
  • 特異性This antibody is specific for MAGED2 (C term).
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human MAGED2.

  • ポジティブ・コントロール
    • Hepatocarcinoma tissue and A549 cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: PC3

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab38491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.

ターゲット情報

  • 関連性MAGED2 is a member of the MAGED gene family. In contrast to the MAGEA and MAGEB genes, which have their entire coding sequences located in the last exon, the MAGED genes have their coding sequences located throughout multiple exons. Also, the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, whereas the MAGED genes are expressed ubiquitously.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • BCG 1 antibody
    • HCA10 antibody
    • JCL 1 antibody
    • MAGE D antibody
    • MAGE D2 antigen antibody
    • Melanoma antigen family D 2 antibody
    • Melanoma associated antigen D2 antibody
    see all

Anti-MAGED2 antibody 画像

  • ICC/IF image of ab38491 stained PC3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38491 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-MAGED2 antibody (ab38491) + A549 lysate

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab38491 at a dilution of 1/50, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-MAGED2 antibody (ab38491) 使用論文

This product has been referenced in:
  • Tseng HY  et al. The melanoma-associated antigen MAGE-D2 suppresses TRAIL receptor 2 and protects against TRAIL-induced apoptosis in human melanoma cells. Carcinogenesis 33:1871-81 (2012). Human . Read more (PubMed: 22791814) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"