製品の概要

  • 製品名Anti-MAGEA4 antibody
    MAGEA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAGEA4
  • 特異性While ab76177 has not been tested for cross-reactivity with other MAGE proteins, the immunogen shows >80% sequence homology with MAGEA1, MAGEA2, MAGEA3, MAGEB3, MAGEA6, MAGEA8, MAGEA9, MAGEA10, and MAGEA12. Based on this information, ab76177 may cross-react with these proteins.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    synthetic peptide from the C-terminus of Human MAGEA4

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • 組織特異性Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • 配列類似性Contains 1 MAGE domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 1.4 antibody
    • CT1.4 antibody
    • MAGA4_HUMAN antibody
    • MAGE 4 antibody
    • MAGE 4 antigen antibody
    • MAGE 41 antibody
    • MAGE 41 antigen antibody
    • MAGE 4A antibody
    • MAGE 4B antibody
    • MAGE X2 antibody
    • MAGE X2 antigen antibody
    • MAGE-4 antigen antibody
    • MAGE-41 antigen antibody
    • MAGE-X2 antigen antibody
    • MAGE4 antibody
    • MAGE41 antibody
    • MAGE4A antibody
    • MAGE4B antibody
    • MAGEA 4 antibody
    • MAGEA4 antibody
    • Melanoma antigen family A 4 antibody
    • Melanoma associated antigen 4 antibody
    • Melanoma-associated antigen 4 antibody
    • MGC21336 antibody
    see all

Anti-MAGEA4 antibody 画像

  • Anti-MAGEA4 antibody (ab76177) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-MAGEA4 antibody (ab76177) 使用論文

This product has been referenced in:
  • Altman E  et al. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues. Reproduction 148:417-27 (2014). Read more (PubMed: 25030892) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

To check the status o...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"