製品の概要

  • 製品名Anti-MAGEA10 antibody
    MAGEA10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAGEA10
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 320-369 (GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE) of human MAGEA10 (NP_001011543).

  • ポジティブ・コントロール
    • Fetal heart lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified by peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. MAGEA10 contains one MAGE domain. Its function is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. MAGEA10 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • 参照データベース
  • 別名
    • Cancer/testis antigen 1.10 antibody
    • cancer/testis antigen family 1, member 10 antibody
    • CT1.10 antibody
    • MAGE 10 antigen antibody
    • MAGE10 antibody
    • Melanoma antigen family A 10 antibody
    • Melanoma associated antigen 10 antibody
    see all

Anti-MAGEA10 antibody 画像

  • Anti-MAGEA10 antibody (ab83557) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

Anti-MAGEA10 antibody (ab83557) 使用論文

ab83557 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"