製品の概要

  • 製品名Anti-MAG antibody
    MAG 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to MAG
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Cow, Cat, Human, Monkey
    交差が予測される動物種: Orangutan
  • 免疫原

    Recombinant fragment: GDLGGYNQYT FSEHSVLDIV NTPNIVVPPE VVAGTEVEVS CMVPDNCPEL RPELSWLGHE GLGEPAVLGR LREDEGTWVQ VSLLHFVPTR, corresponding to amino acids 119-208 of Human MAG (NP_002352) with a proprietary tag.

  • ポジティブ・コントロール
    • Partial tagged recombinant Human MAG protein (Immunogen), Jurkat cell lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/400.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/2000 - 1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa. Isoform a, 69 kDa; Isoform b, 63 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
  • 配列類似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GMA antibody
    • MAG antibody
    • MAG_HUMAN antibody
    • Myelin associated glycoprotein antibody
    • Myelin-associated glycoprotein antibody
    • S MAG antibody
    • S-MAG antibody
    • Sialic acid binding Ig like lectin 4A antibody
    • Sialic acid binding immunoglobulin like lectin 4A antibody
    • Siglec 4a antibody
    • Siglec-4a antibody
    • SIGLEC4A antibody
    • SPG75 antibody
    see all

Anti-MAG antibody 画像

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab89780 staining MAG in lamb spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/5000 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab89780 staining MAG in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100) at 4°C for 18 hours. An Alexa Fluor® 594-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC-P image of MAG staining on cow peripheral nerve sections using ab89780 (1:4000). The sections were fixed in Formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 mins at 21°C.

    See Abreview

  • Anti-MAG antibody (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG protein at 0.2 µg

    Predicted band size : 63 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.
  • Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MAG antibody (ab89780) 使用論文

This product has been referenced in:
  • Wahl SE  et al. Mammalian target of rapamycin promotes oligodendrocyte differentiation, initiation and extent of CNS myelination. J Neurosci 34:4453-65 (2014). Mouse . Read more (PubMed: 24671992) »
  • Bercury KK  et al. Conditional ablation of raptor or rictor has differential impact on oligodendrocyte differentiation and CNS myelination. J Neurosci 34:4466-80 (2014). Read more (PubMed: 24671993) »

See all 8 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Specification Sciatic nerve
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2016

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Specification Sciatic nerve
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Sheep Tissue sections (Lamb spinal cord)
Specification Lamb spinal cord
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - methanol
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Sep 12 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10% Gel)
Sample Rat Tissue lysate - whole (cerebral cortex)
Specification cerebral cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Sep 11 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Lizard Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 18 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Sciatic nerve)
Loading amount 30 µg
Specification Sciatic nerve
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

投稿 May 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain)
Specification brain
Fixative Formaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

投稿 Nov 06 2012

Thank you for contacting us.

We do not know whether this antibody will recognize S- and/or L-MAG as this has not been specifically tested in our lab. I don't know much about the differences between S- and L-MAG, but in theory if they contain...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"