製品の概要

  • 製品名Anti-MAFK antibody - ChIP Grade
    MAFK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAFK - ChIP Grade
  • 特異性Seven amino acids are identical in MaFG in the C-terminal region of the peptide.
  • アプリケーション適用あり: ChIP, IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide:

    TTNPKPNKALKVKKEA

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 2-17 of Human MAFK

  • ポジティブ・コントロール
    • Whole extracts of the A431 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP
IP
WB
ICC/IF
  • 追加情報IP: Use 4 - 8 µl immunoprecipitates MAFF from the A431 cell line.

    WB: 1/4000 - 1/8000. Predicted molecular weight: 17 kDa. Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20.
    Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
    • 配列類似性Belongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Erythroid transcription factor NF E2 p18 subunit antibody
      • Erythroid transcription factor NF-E2 p18 subunit antibody
      • FLJ32205 antibody
      • MAFK antibody
      • MAFK_HUMAN antibody
      • MGC71717 antibody
      • Nfe2u antibody
      • P18 antibody
      • Transcription factor MafK antibody
      • V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) antibody
      see all

    Anti-MAFK antibody - ChIP Grade 画像

    • ChIP analysis using ab50322 binding MAFK in Human primary macrophage. Cells were cross-linked with formaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. Protein binding was detected using real-time PCR.

       

      See Abreview

    • Lanes 1 & 3 : Anti-MAFK antibody - ChIP Grade (ab50322) at 1/4000 dilution
      Lane 2 : Anti-MAFK antibody - ChIP Grade (ab50322) at 1/8000 dilution
      Lane 4 : no primary antibody

      Lane 1 : A431 whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : A431 whole cell lysate with MAFF immunizing peptide at 10 µg
      Lane 4 : A431 whole cell lysate

      Secondary
      Goat Anti-Rabbit IgG, Peroxidase conjugate

      Predicted band size : 17 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Anti-MAFK antibody - ChIP Grade (ab50322) 使用論文

    This product has been referenced in:
    • Marinov GK  et al. Evidence for site-specific occupancy of the mitochondrial genome by nuclear transcription factors. PLoS One 9:e84713 (2014). ICC/IF ; Human . Read more (PubMed: 24465428) »
    • Hwang YJ  et al. Oleanolic acid regulates NF-?B signaling by suppressing MafK expression in RAW 264.7 cells. BMB Rep 47:524-9 (2014). Read more (PubMed: 25059280) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (macrophage)
    Specification macrophage
    Treatment 0.1 ug/ml LPS 15 min
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 24 2014

    Application ChIP
    Detection step Real-time PCR
    Sample Human Cell lysate - whole cell (primary human macrophage)
    Specification primary human macrophage
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jan 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"