製品の概要
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製品名Anti-MafB antibody
MafB 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to MafB
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Zebrafish
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免疫原
Synthetic peptide corresponding to a region of Human MAFB
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human MafB protein
- HepG2 cell lysate; human spleen tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab56242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
ターゲット情報
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機能Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
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組織特異性Ubiquitous.
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関連疾患Defects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
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配列類似性Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
ドメインThe leucine-zipper domain is involved in the interaction with LRPICD.
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翻訳後修飾Phosphorylated by GSK3 and MAPK13 on serine and threonine residues.
Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 9935 Human
- Entrez Gene: 16658 Mouse
- Entrez Gene: 54264 Rat
- Entrez Gene: 30210 Zebrafish
- GenBank: AF134157.1 Human
- Omim: 608968 Human
- SwissProt: Q9Y5Q3 Human
- SwissProt: P54841 Mouse
see all -
別名
- Kreisler antibody
- Kreisler (mouse) maf related leucine zipper homolog antibody
- Kreisler maf related leucine zipper homolog antibody
see all
Anti-MafB antibody 画像
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Western blot - MafB antibody (ab56242)Anti-MafB antibody (ab56242) at 0.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP-conjugated anti-rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Gel concentration 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody (ab56242)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling MafB with ab56242 at 4-8µg/ml. Arrows indicate positively labelled spleen cells. Magnification 400X.
Anti-MafB antibody (ab56242) 使用論文
This product has been referenced in:
- Gäken J et al. A functional assay for microRNA target identification and validation. Nucleic Acids Res 40:e75 (2012). WB ; Human . Read more (PubMed: 22323518) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"