製品の概要

  • 製品名Anti-MAFA antibody
    MafA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAFA
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 300-359 of Mouse MafA (NP_919331.1).

  • ポジティブ・コントロール
    • Mouse Pancreas tissue.
  • 特記事項

    Concentration is optimized for IHC and not determined

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer or tris-EDTA pH9 buffer will enhance staining. Extended incubation with primary antibody (16-24 hours) at room temperature is recommended.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
    • 配列類似性Belongs to the bZIP family. Maf subfamily.
      Contains 1 bZIP domain.
    • 翻訳後修飾Ubiquitinated, leading to its degradation by the proteasome.
      Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
    • 細胞内局在Nucleus. Detected in nuclei of pancreas islet beta cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HMafA antibody
      • Mafa antibody
      • MAFA_HUMAN antibody
      • Pancreatic beta cell specific transcriptional activator antibody
      • Pancreatic beta-cell-specific transcriptional activator antibody
      • RIPE3b1 antibody
      • Transcription factor MafA antibody
      • Transcription factor RIPE3b1 antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
      • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
      see all

    Anti-MAFA antibody 画像

    • ab85008 at 1/250 dilution staining MafA in mouse pancreatic islet by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    Anti-MAFA antibody (ab85008) 使用論文

    ab85008 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"