Anti-MAdCAM1 抗体 [AP-MAB0842] (ab80680)

製品の概要

  • 製品名Anti-MAdCAM1 antibody [AP-MAB0842]
    MAdCAM1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [AP-MAB0842] to MAdCAM1
  • アプリケーション適用あり: IHC-P, Inhibition Assay, IP, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse Endothelial cells

  • 特記事項
    Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).


    ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
    ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Inhibition Assay Use at an assay dependent concentration.

ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.

IP 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/200 - 1/400.

ターゲット情報

  • 機能Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • 組織特異性Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • 配列類似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻訳後修飾The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

Anti-MAdCAM1 antibody [AP-MAB0842] 画像

  • ab80680 staining MAdCAM1 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate, pH 6.0. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA) for 30 minutes. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-MAdCAM1 antibody [AP-MAB0842] (ab80680) 使用論文

ab80680 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
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Mr. Alex Van Engelenburg

Verified customer

投稿 Jul 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"