製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAD4
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: DFAREKTKAA GLVRKAPNNR SSHNELEKHR RAKLRLYLEQ LKQLVPLGPD, corresponding to N terminal amino acids 36-85 of Human MAD4

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 - 2.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
  • 配列類似性
    Contains 1 bHLH (basic helix-loop-helix) domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHc12 antibody
    • Class C basic helix loop helix protein 12 antibody
    • Class C basic helix-loop-helix protein 12 antibody
    • MAD 4 antibody
    • MAD4 antibody
    • Mad4 homolog antibody
    • MAD4_HUMAN antibody
    • Max associated protein 4 antibody
    • MAX dimerization protein 4 antibody
    • Max dimerizer 4 antibody
    • Max interacting transcriptional repressor MAD4 antibody
    • Max-associated protein 4 antibody
    • Max-interacting transcriptional repressor MAD4 antibody
    • MST 149 antibody
    • MST149 antibody
    • MSTP 149 antibody
    • MSTP149 antibody
    • MXD 4 antibody
    • MXD4 antibody
    see all

画像

  • All lanes : Anti-MAD4 antibody (ab66367) at 0.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 30,48 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab66367 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (foreskin)
Permeabilization
No
Specification
foreskin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
none
Username

Mr. Julien Coutier

Verified customer

投稿 Jul 05 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Primary keratinocytes from skin epithelium)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Primary keratinocytes from skin epithelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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