製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Mad2L2
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    TLTRQDLNFGQV

    conjugated to KLH, corresponding to amino acids 3-14 of Human Mad2L2 (NP_006332.2).

  • ポジティブ・コントロール
    • Human Brain, Cerebellum tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.Can be blocked with Human Mad2L2 peptide (ab11254).
ELISA 1/7500 - 1/30000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Contains 1 HORMA domain.
  • 細胞内局在
    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homolog of REV7 S cerevisiae antibody
    • hREV7 antibody
    • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
    • MAD2 homolog antibody
    • MAD2 like 2 antibody
    • MAD2 mitotic arrest deficient like 2 antibody
    • MAD2-like protein 2 antibody
    • MAD2B antibody
    • Mad2l2 antibody
    • MD2L2_HUMAN antibody
    • Mitotic Arrest Deficient 2 L2 antibody
    • Mitotic arrest deficient 2-like protein 2 antibody
    • Mitotic arrest deficient homolog like 2 antibody
    • Mitotic arrest deficient like 2 (yeast) antibody
    • Mitotic arrest deficient yeast homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD2B antibody
    • Polymerase (DNA directed) zeta 2 accessory subunit antibody
    • POLZ2 antibody
    • REV 7 antibody
    • REV7 antibody
    • REV7 homolog antibody
    • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
    see all

Anti-Mad2L2 antibody 画像

  • All lanes : Anti-Mad2L2 antibody (ab115622) at 1/1000 dilution

    Lane 1 : Mouse E10.5 whole embryo lysate
    Lane 2 : Mouse E12.5 whole embryo lysate
    Lane 3 : Mouse E17.5 whole brain lysate
    Lane 4 : HeLa cell lysate

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab115622 staining Mad2L2 (green) in Human lung cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with the Hope technique. Samples were incubated with primary antibody (1/300) for 30 minutes at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Right - negative control.

    See Abreview

  • ab115622 staining Mad2L2 in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the Hope Technique and blocked for 15 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab115622, at 10µg/ml, staining Mad2L2 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Mad2L2 antibody (ab115622) 使用論文

ab115622 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
0 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (E12.5 whole embryo lysate)
Specification
E12.5 whole embryo lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (lung cancer tissue)
Specification
lung cancer tissue
Permeabilization
No
Fixative
Hope-Technique
Username

Abcam user community

Verified customer

投稿 Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Zytomed Systems HRP Polymer Kit Blocking Solution as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (Lung cancer)
Specification
Lung cancer
Permeabilization
No
Fixative
Hope-Technique
Username

Abcam user community

Verified customer

投稿 Jun 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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