Anti-Mad2L1 抗体 [17D10] (ab10691)
Key features and details
- Mouse monoclonal [17D10] to Mad2L1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Mad2L1 antibody [17D10]
Mad2L1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [17D10] to Mad2L1 -
由来種
Mouse -
特異性
Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant human Mad2.
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ポジティブ・コントロール
- WB: HAP1, HeLa and A431 cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from supernatant. -
ポリ/モノ
モノクローナル -
クローン名
17D10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10691の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. -
配列類似性
Belongs to the MAD2 family.
Contains 1 HORMA domain. -
ドメイン
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. -
翻訳後修飾
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. - Information by UniProt
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参照データベース
- Entrez Gene: 4085 Human
- Omim: 601467 Human
- SwissProt: Q13257 Human
- Unigene: 591697 Human
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別名
- HsMAD 2 antibody
- HsMAD2 antibody
- MAD 2 antibody
see all
画像
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All lanes : Anti-Mad2L1 antibody [17D10] (ab10691) at 2 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Mad2L1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 24.6 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab10691 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab10691 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab10691 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Western blot using ab10691 at 1/500.
Lane 1: Hela Nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Mad2L1 predicted size: 24.6kD.
Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
Exposure time: 1min
20µg of lysate per lane.Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.
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Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.
Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).
Blocking Step: 5% Milk for 16 hours at 4°C
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab10691 は 9 報の論文で使用されています。
- Tischer T et al. The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly. J Cell Sci 135:N/A (2022). PubMed: 34878135
- Li Q et al. CKAP2L, a crucial target of miR-326, promotes prostate cancer progression. BMC Cancer 22:666 (2022). PubMed: 35715760
- Villarroya-Beltri C et al. Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility. Sci Adv 8:eabq5914 (2022). PubMed: 36322655
- Lu S et al. Bioinformatics Analysis and Validation Identify CDK1 and MAD2L1 as Prognostic Markers of Rhabdomyosarcoma. Cancer Manag Res 12:12123-12136 (2020). PubMed: 33273853
- Alfieri C et al. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559:274-278 (2018). PubMed: 29973720
- Tambe M et al. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel. Oncotarget 7:12267-85 (2016). WB . PubMed: 26943585
- Gysin S et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012). WB ; Human . PubMed: 22833572
- Sarfraz S et al. Modulations of cell cycle checkpoints during HCV associated disease. BMC Infect Dis 9:125 (2009). WB ; Human . PubMed: 19664251
- Wise SS et al. Chronic exposure to particulate chromate induces spindle assembly checkpoint bypass in human lung cells. Chem Res Toxicol 19:1492-8 (2006). WB ; Human . PubMed: 17112237