製品の概要

  • 製品名Anti-MAD2 antibody
    MAD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAD2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant bacterial His6-fusion protein (S. cerevisiae).

  • ポジティブ・コントロール
    • S.cerevisiae nuclear extracts (see image below).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100. Detects a band of approximately 23 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • 配列類似性Belongs to the MAD2 family.
    Contains 1 HORMA domain.
  • ドメインThe protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
  • 翻訳後修飾Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
  • Information by UniProt
  • 別名
    • HsMAD2 antibody
    • MAD 2 antibody
    • MAD2 like 1 antibody
    • MAD2 mitotic arrest deficient like 1 antibody
    • MAD2-like protein 1 antibody
    • Mad2l1 antibody
    • MD2L1_HUMAN antibody
    • Mitotic arrest deficient 2-like protein 1 antibody
    • Mitotic spindle assembly checkpoint protein MAD2A antibody
    • REV7 antibody
    see all

Anti-MAD2 antibody 画像

  • ab11690 at a 1/100 dilution staining approximately 23kDa S.cerevisiae MAD2 in nucleoplasm extract, nuclear membrane extract and whole nuclear extract by Western blot (ECL).

Anti-MAD2 antibody (ab11690) 使用論文

This product has been referenced in:

See 1 Publication for this product

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