Synthetic peptide designed within residues: PQKFIVDYSE TSPQCPKPGV ILLTKRGRQI CADPNKKWVQ KYISDLKLNA, corresponding to internal sequence amino acids 40-89 of Human Macrophage Inflammatory Protein 4 (NP_002979).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (use citrate buffer pH6.0)
Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
Belongs to the intercrine beta (chemokine CC) family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Macrophage Inflammatory Protein 4 with ab104867 at 4-8µg/ml. Arrows inducate positively labelled epithelial cells of intestinal villi. Magnification: 400X.
Muñoz-Esquerre M et al. Vascular disease in COPD: Systemic and pulmonary expression of PARC (Pulmonary and Activation-Regulated Chemokine). PLoS One12:e0177218 (2017).
Read more (PubMed: 28545096) »
Furukawa S et al. Preferential M2 macrophages contribute to fibrosis in IgG4-related dacryoadenitis and sialoadenitis, so-called Mikulicz's disease. Clin Immunol156:9-18 (2015).
Read more (PubMed: 25450336) »