Anti-Macrophage Inflammatory Protein 4 抗体 (ab104867)

製品の概要

  • 製品名Anti-Macrophage Inflammatory Protein 4 antibody
    Macrophage Inflammatory Protein 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Macrophage Inflammatory Protein 4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide designed within residues: PQKFIVDYSE TSPQCPKPGV ILLTKRGRQI CADPNKKWVQ KYISDLKLNA, corresponding to internal sequence amino acids 40-89 of Human Macrophage Inflammatory Protein 4 (NP_002979).

  • ポジティブ・コントロール
    • Human fetal thymus lysate; Human intestine tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (use citrate buffer pH6.0)

ターゲット情報

  • 機能Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
  • 組織特異性Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alternative macrophage activation associated CC chemokine 1 antibody
    • Alternative macrophage activation-associated CC chemokine 1 antibody
    • AMAC-1 antibody
    • AMAC1 antibody
    • CC chemokine PARC antibody
    • CCL18 antibody
    • CCL18(4-69) antibody
    • CCL18_HUMAN antibody
    • CKb7 antibody
    • DC-CK1 antibody
    • DCCK1 antibody
    • DCCK1 antibody
    • Dendritic cell chemokine 1 antibody
    • Macrophage inflammatory protein 4 antibody
    • MIP-4 antibody
    • MIP4 antibody
    • PARC antibody
    • Pulmonary and activation regulated chemokine antibody
    • Pulmonary and activation-regulated chemokine antibody
    • SCYA18 antibody
    • Small inducible cytokine A18 antibody
    • Small inducible cytokine A18 antibody
    • Small-inducible cytokine A18 antibody
    see all

Anti-Macrophage Inflammatory Protein 4 antibody 画像

  • Anti-Macrophage Inflammatory Protein 4 antibody (ab104867) at 0.5 µg/ml + Human fetal thymus lysate at 10 µg

    Predicted band size : 10 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Macrophage Inflammatory Protein 4 with ab104867 at 4-8µg/ml. Arrows inducate positively labelled epithelial cells of intestinal villi. Magnification: 400X.

Anti-Macrophage Inflammatory Protein 4 antibody (ab104867) 使用論文

This product has been referenced in:
  • Furukawa S  et al. Preferential M2 macrophages contribute to fibrosis in IgG4-related dacryoadenitis and sialoadenitis, so-called Mikulicz's disease. Clin Immunol 156:9-18 (2015). IHC-P ; Human . Read more (PubMed: 25450336) »
  • Chen G  et al. CC Chemokine Ligand 18 Correlates with Malignant Progression of Prostate Cancer. Biomed Res Int 2014:230183 (2014). IHC-P ; Human . Read more (PubMed: 25197632) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (human Lung homogenate)
Gel Running Conditions Reduced Denaturing (Gel Tris-Glicine 4-20% poliacrilamide)
Loading amount 40 µg
Treatment boil the samples 10 min at 58¹C with Laemmli 4X+5% betamercaptoetanol before loading samples into the gel
Specification human Lung homogenate
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 20 2017

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung)
Permeabilization Yes - 0.1 % triton X-100
Specification Lung
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Elisabeth Aliagas

Verified customer

投稿 Jun 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"