Anti-Macrophage Inflammatory Protein 1 beta 抗体 (ab10386)

製品の概要

  • 製品名Anti-Macrophage Inflammatory Protein 1 beta antibody
    Macrophage Inflammatory Protein 1 beta 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Macrophage Inflammatory Protein 1 beta
  • 特異性Mouse MIP1 beta neutralizes the bioactivity of recombinant, mouse MIP1 beta, but not that of recombinant, human MIP1 beta, recombinant, human MIP1 alpha or recombinant, mouse MIP1 alpha. In indirect ELISA and immunoblotting, this antibody displays less than 2% cross-reactivity with recombinant, mouse MIP1 alpha, recombinant, human MIP1 alpha and recombinant, human MIP1 alpha. The product shows no cross-reactivity with other tested cytokines using indirect ELISA.
  • アプリケーション適用あり: ELISA, WB, Neutralisingmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse recombinant macrophage inflammatory protein-1beta, expressed in E. coli, as the immunogen.

  • 特記事項Endotoxin: <10 ng/vial.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
Neutralising
  • 追加情報ELISA: Use at a concentration of 0.5 µg/ml to detect <1ng/well of recombinant mouse MIP1 beta.
    Neut: Use at a concentration of 50 - 100 µg/ml.
    WB: Use at a concentration of 1 µg/ml to detect recombinant mouse MIP1 beta at 5ng/lane under reducing conditions.
    Predicted molecular weight: 11 kDa.

    Anti-Mouse MIP1 beta was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 beta in an in vitro colony forming assay using hematopoietic stem cells.

    The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, mouse MIP1 beta, which is present at five times its own EC50 (the concentration of recombinant, mouse MIP1 beta producing a one-half maximal bioactivity without antibody).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
    • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • MIP 1 beta antibody
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      • CC chemokine ligand 4L2 antibody
      • CCL4 antibody
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      • ccl4l 1 antibody
      • CCL4L antibody
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      • Chemokine (C C motif) ligand 4 antibody
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      • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
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      • Chemokine CC Motif Ligand 4 antibody
      • G 26 antibody
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      • G-26 T-lymphocyte-secreted protein antibody
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      • MIP-1-beta(3-69) antibody
      • MIP1 beta antibody
      • MIP1B antibody
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      • SCYA2 antibody
      • SCYA4 antibody
      • SCYA4L antibody
      • SCYA4L1 antibody
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      • Secreted protein G 26 antibody
      • Secreted protein G26 antibody
      • SIS gamma antibody
      • SIS-gamma antibody
      • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
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      • small inducible cytokine A4-like antibody
      • Small-inducible cytokine A4 antibody
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      see all

    Anti-Macrophage Inflammatory Protein 1 beta antibody (ab10386) 使用論文

    This product has been referenced in:
    • Johannsen A  et al. Definition of Key Variables for the Induction of Optimal NY-ESO-1-Specific T Cells in HLA Transgene Mice. J Immunol 185:3445-55 (2010). Neutralising ; Mouse . Read more (PubMed: 20733200) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"