製品の概要

  • 製品名Anti-Macrophage antibody [PM-2K]
    Macrophage 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PM-2K] to Macrophage
  • 特異性Does not recognize Macrophage Scavenger Receptor AI or AII as determined using positive CHO cells.
  • アプリケーション適用あり: WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Cow, Cat, Dog, Human, Pig, Monkey
    非交差種: Rat, Rabbit, Goat, Horse, Guinea pig, Hamster
  • 免疫原

    Cultured human peritoneal macrophages.

  • ポジティブ・コントロール
    • Macrophage cell membranes.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA, pH 7.2
  • Concentration information loading...
  • 特記事項(精製)Purified.
  • ポリ/モノモノクローナル
  • クローン名PM-2K
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/800.
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • 別名
    • macrophages antibody

Anti-Macrophage antibody [PM-2K] (ab58822) 使用論文

This product has been referenced in:
  • Marshall DC  et al. Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal Cancer. PLoS One 10:e0127063 (2015). IHC . Read more (PubMed: 25961845) »
  • Tsai YM  et al. Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes. PLoS One 8:e64105 (2013). Flow Cyt ; Human . Read more (PubMed: 23734186) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Permeabilization No
Sample Human Cell (peripheral blood)
Specification peripheral blood
Gating Strategy Gated on CD45+PM2K+ cells
Preparation Cell harvesting/tissue preparation method: The blood samples are centrifuged at 2500RPM for 10 mins, and lyzed with ACK buffer 2X, 5 mins each and suspended in FA buffer
Sample buffer: FA buffer (1X PBS+0.2% FBS)
Username

Dr. Yunge Zhao

Verified customer

投稿 Jun 19 2013

Thank you for contacting Abcam.

The expected band size is around 150kDA. I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol including the tissue...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"