製品の概要

  • 製品名Anti-Macrophage antibody [MAC387]
    Macrophage 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MAC387] to Macrophage
  • 特異性Recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey
    交差が予測される動物種: Horse, Guinea pig, Cat, Baboon非交差種: Rat
  • 免疫原

    Tissue/ cell preparation (Human). Human monocytes

  • ポジティブ・コントロール
    • Human spleen

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium azide
    Constituent: PBS

    Some batches may contain 1% BSA. Please contact Scientific Support for batch information.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノモノクローナル
  • クローン名MAC387
  • ミエローマNS1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

Membrane permeabilisation is required for this application.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/100 - 1/200.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • 別名
    • macrophages antibody

Anti-Macrophage antibody [MAC387] 画像

  • Overlay histogram showing THP1 cells stained with ab22506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22506, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab22506 staining Macrophage in 12um frozen sections of pig skin by Immunohistochemistry (IHC-Fr - frozen sections). Tissue permeabilized with 0..25% Tween20 in TBS and blocked with 5% serum for 60 minutes at 20°C. Samples were incubated with primary antibody (1/200 in TBS 0.02% Triton X-100, 1% BSA) for 16 hours at 4°C. An Alexa®488-conjugated Donkey anti-mouse IgG monoclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.

    See Abreview

  • IHC image of Macrophage staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab22506 staining Macrophages in human brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone and blocked for 30 minutes at 25°C. The primary antibody was diluted, 1/100 and incubated with sample for 1 hours at 37°C. A Biotin conjugated goat polyclonal to mouse IgG1, diluted 1/250 was used as secondary. The Biotinylated sites were visualized with Invitrogen streptavidin-Alexa546.

    See Abreview

  • IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Macrophage antibody [MAC387] (ab22506) 使用論文

This product has been referenced in:
  • Tarkia M  et al. [18F]FDG Accumulation in Early Coronary Atherosclerotic Lesions in Pigs. PLoS One 10:e0131332 (2015). Read more (PubMed: 26120829) »
  • Sun H  et al. Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor. J Cell Mol Med 19:836-49 (2015). IHC-P ; Mouse . Read more (PubMed: 25661015) »

See all 21 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Pig Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Pig Tissue sections (Skin)
Specification Skin
Permeabilization Yes - 0.05% Tween 20
Fixative 10% Formalin
Username

Abcam user community

Verified customer

投稿 Oct 28 2014

L1 is as an alternative name for Calprotectin. I was also able to locate the correct Swiss Prot Id for you: P05109 (S10A8_HUMAN) http://www.uniprot.org/uniprot/P05109

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Spleen and Aortic Valve Tissue)
Specification Spleen and Aortic Valve Tissue
Fixative 10% NBF
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin-CaCl 0.001 mg/mL, 30 min at 37*C
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Krista Sider

Verified customer

投稿 Jan 21 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (normal and wounded skin)
Specification normal and wounded skin
Fixative no fixation
Permeabilization Yes - 0.25% tween 20 in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 15 2012

Thank you for your enquiry and your interest in our products.

I can confirm that ab22506 has only been tested in Mouse, Rabbit, Cow, Dog, Human, Pig, Monkey and due to sequence homology it may work in Horse, Guinea pig, Cat, Baboon. There are...

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Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab56297 on the order ***, which should arrive tomorrow.

Please keep me updated about t...

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1-10 of 38 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"