Anti-Macrophage 抗体 [HIS36] (Phycoerythrin) (ab95574)

製品の概要

  • 製品名Anti-Macrophage antibody [HIS36] (Phycoerythrin)
    Macrophage 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIS36] to Macrophage (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Rat bone marrow cells.

  • ポジティブ・コントロール
    • Rat spleen and peritoneal cells
  • 特記事項


    The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of Rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate elicited peritoneal cells and tissue macrophages. ab95574 is used as a marker for Rat tissue macrophages.
  • ポリ/モノモノクローナル
  • クローン名HIS36
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.25-0.5µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.



ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
  • 別名
    • macrophages antibody

Anti-Macrophage antibody [HIS36] (Phycoerythrin) 画像

  • ab95574, at 0.25 µg per test, staining Rat thioglycolate induced peritoneal exudate cells (PECs) by flow cytometry (filled histogram). The open histogram shows staining with 0.25 µg of Mouse IgG2a ? Isotype Control PE. Total viable cells were used for analysis.

Anti-Macrophage antibody [HIS36] (Phycoerythrin) (ab95574) 使用論文

ab95574 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"