Application
Flow Cytometry
Sample
Mouse Cell (Embryonic Stem Cells)
Permeabilization
Yes - Saponin
Gating Strategy
life ES cell gate, excluding debris and MEFs
Specification
Embryonic Stem Cells
Preparation
Cell harvesting/tissue preparation method: feeder free single cell suspension via trypsination and gelatine culture for 1h
Sample buffer: PBS 0.3% Saponin
Sample buffer: PBS 0.3% Saponin
Fixation
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS 0.3% Saponin
Concentration
1 µg/cells
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit-PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Nadine Obier
Verified customer
投稿 Apr 28 2008