Anti-Macro H2A.2 抗体 (ab102126)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Macro H2A.2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 197-340 of Human Macro H2A.2 (AAH16172).

  • ポジティブ・コントロール
    • A431 whole cell lysate, TOV-21G xenograft

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, PBS, pH 7
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

ターゲット情報

  • 機能
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
  • 配列類似性
    Contains 1 histone H2A domain.
    Contains 1 Macro domain.
  • 細胞内局在
    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Core histone macro H2A2.2 antibody
    • Core histone macro-H2A.2 antibody
    • Core histone macroH2A2.2 antibody
    • H2A histone family member Y2 antibody
    • H2AFY2 antibody
    • H2AW_HUMAN antibody
    • Histone macroH2A2 antibody
    • Macro H2A2 antibody
    • MacroH2A.2 antibody
    • MacroH2A2 antibody
    • mH2A2 antibody
    see all

画像

  • Anti-Macro H2A.2 antibody (ab102126) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

参考文献

ab102126 has not yet been referenced specifically in any publications.

レビューと Q&A

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Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Brain nuclei)
Negative control
IgG
Specification
Brain nuclei
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.5% Fromaldehyde
Positive control
H3
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投稿 Feb 10 2017

Application
ChIP
Sample
Mouse Cell lysate - whole cell (ES)
Specification
ES
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
No
Negative control
macroH2a2-/- ES cells
IgG ab
Username

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Verified customer

投稿 Nov 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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