製品の概要

  • 製品名
    Anti-MACF1 antibody
  • 製品の詳細
    Rabbit polyclonal to MACF1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 1-50 of Mouse MACF1.

  • ポジティブ・コントロール
    • Mouse kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: Proprietary component, Whole serum
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab117418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 832 kDa.
ELISA 1/10000.

ターゲット情報

  • 関連性
    Microtubule-actin crosslinking factor 1 (MACF1/ACF7) belongs to spectraplakin family of proteins which also includes mammalian Bpag1/dystonin, Drosophila Short Stop (Shot) and Caenorhabditis elegans Vab-10. These are large actin-microtubule linker molecules that are essential for various biological processes such as neuronal degeneration, wound healing, gastrulation. MACF1, located on mouse chromosome 4D2.2, was found to play an important role during neurogenesis- it was shown that MACF1 regulates the organization of neuronal microtubules which is essential for axon extension. Furthermore, MACF1 is required in controlling focal adhesion assembly and dynamics. MACF1 behaves as an actin-regulated ATPase and it has the ability to target microtubules that track along F-actin to focal adhesions. MACF1 was also implicated in regulating filopodia formation.
  • 細胞内局在
    Cytoplasmic, microtubule. Plasma membrane. Golgi apparatus.
  • 参照データベース
  • 別名
    • ABP620 antibody
    • Acf7 antibody
    • Aclp7 antibody
    • Actin cross linking family 7 antibody
    • MACF antibody
    • Microtubule actin crosslinking factor 1 antibody
    • mKIAA0465 antibody
    • R74989 antibody
    • rabeculin alpha antibody
    see all

画像

  • ab117418 at 1/500 dilution staining MACF1 in Mouse kidney tissue by Immunofluorescence.

プロトコール

参考文献

ab117418 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for sending the protocol again. This time it arrived with the message.

The first thing I recommend checking is whether the transfer worked. You can do this with a Ponceau Red stain, before you incubate with the antibody. The protei...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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