Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
1000 µg
Specification
293T
Immuno-precipitation step
Protein G
Other product details
Dilution
1/333
Incubation time
1 hour(s) and 20 minute(s) · Temperature: 4°C
Western Blot antibody
Name
Non-Abcam antibody was used: mTOR CST#2972, rictor CST#2140, raptor CST#4978
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Additional data
Additional Notes
Appears to IP mTOR and rictor at low amounts, but not raptor.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 05 2010