Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X-100 for 1 minute
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal donkey serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Additional Notes
Nice signal but have NOT validated with mTOR knockdown in these cells. Unknown if staining is non-specific.
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投稿 Mar 24 2010