Application
IHC - Wholemount
Sample
Zebrafish Embryo (To detect exogenous mCherry expression in brain)
Specification
To detect exogenous mCherry expression in brain
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10%goat serum,1%DMSO,0.5%TritonX in PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L), highly
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
5 days post fertilisation embryos have been fixed for 4 hours at room temperature in 4% paraformaldehyde / 4% sucrose in PBS.
The skin of the embryos has been dissected for better accessibility of deeper brain tissues.
Embryos were further permeabilised in a 10ug/mL proteinase K solution in PBS+0.5%TritonX, for 5 minutes at room temperature.
Abcam response
Thank you for submitting an Abreview. IHC- Wholemount was not a tested application for ab125096 and apparently it does not seem to work well in this experimental procedure, under these conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 20 2013