Anti-mCherry 抗体 [1C51] (ab125096)
Key features and details
- Mouse monoclonal [1C51] to mCherry
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG2a
製品の概要
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製品名
Anti-mCherry antibody [1C51]
mCherry 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C51] to mCherry -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Recombinant full length protein corresponding to mCherry.
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ポジティブ・コントロール
- IHC-P: mCherry-transfected monkey brain tissue, Drosophila melanogaster eye imaginal discs and smooth muscle cells in rat bladder tissue. WB: HEK-293 cells transfected with mCherry-HA construct and mCherry recombinant protein. ICC/IF: mCherry-transfected HEK-293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
1C51 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125096の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/2000. Detects a band of approximately 28 kDa.
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ICC/IF | (3) |
1/500.
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IHC-P | (4) |
1/2000.
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特記事項 |
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WB
1/2000. Detects a band of approximately 28 kDa. |
ICC/IF
1/500. |
IHC-P
1/2000. |
ターゲット情報
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関連性
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
画像
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Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, dilution 1/2000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. ab125096 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model.
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4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.
Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.
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All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HEK-293 cells transfected with mCherry-HA construct
Lane 4 : mCherry recombinant protein
Lane 5 : GFP recombinant protein
Lane 6 : HEK-293 transfected with GFP construct.Major band at about 30kDa corresponds to mCherry protein.
MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
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mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.
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ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (175)
ab125096 は 175 報の論文で使用されています。
- Xia B et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2. Cell Discov 9:2 (2023). PubMed: 36609376
- Quintanilla CG et al. Nir1 constitutively localizes at ER-PM junctions and promotes Nir2 recruitment for PIP2 homeostasis. Mol Biol Cell 33:br2 (2022). PubMed: 35020418
- Liu Y et al. Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene. Gene Ther 29:368-378 (2022). PubMed: 35383288
- Shkarina K et al. Optogenetic activators of apoptosis, necroptosis, and pyroptosis. J Cell Biol 221:N/A (2022). PubMed: 35420640
- Ferrandiz N et al. Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. J Cell Biol 221:N/A (2022). PubMed: 35486148