Anti-M6PR (cation independent) 抗体 [MEM-238] (FITC) (ab58841)


  • 製品名
    Anti-M6PR (cation independent) antibody [MEM-238] (FITC)
    M6PR (cation independent) 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-238] to M6PR (cation independent) (FITC)
  • 由来種
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: ICC/IF, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Non human primates
  • 免疫原

    Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • エピトープ
    ab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).
  • ポジティブ・コントロール
    • Jurkat whole cell lysate
  • 特記事項

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.



Our Abpromise guarantee covers the use of ab58841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50.
WB Use at an assay dependent concentration. Predicted molecular weight: 275 kDa. (non-reducing conditions).
Flow Cyt Use 8.3µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • 機能
    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • 配列類似性
    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • ドメイン
    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • 細胞内局在
    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all


  • ab58841 staining Mannose 6 Phosphate Receptor in a Human cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% NP-40 + 1% BSA in PBS and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/50 in 0.5% NP-40 + 1% BSA in PBS) for 1 hour at 25°C.

    See Abreview

  • Lane 1 : Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)
    Lane 2 : Mouse IgG1 isotype control

    All lanes : Jurkat whole cell lysate

    Predicted band size: 275 kDa

  • Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.


This product has been referenced in:
  • Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhibition Assay ; Human . Read more (PubMed: 19414391) »
  • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt ; Human . Read more (PubMed: 12189157) »

See all 2 Publications for this product

レビューと Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Human Cell (HEK293, HeLa)
HEK293, HeLa
Yes - 0.5% NP-40, 1%BSA in PBS

Dr. Monica Pons

Verified customer

投稿 Oct 01 2013

Thank you for contacting us. The following two papers include protocols with this clone, MEM-238, which indicate that the antibody recognizes an extracellular epitope. This will also be the case for the FITC conjugate, ab58441. (The unconjugated versio...

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