Anti-M6PR (cation independent) 抗体 - Lysosome Membrane Marker (ab12894)

製品の概要

  • 製品名
    Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker
    M6PR (cation independent) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to M6PR (cation independent) - Lysosome Membrane Marker
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human, African green monkey
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from residues 700 - 800 of Human Mannose 6 Phospate Receptor.

    .

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.

ターゲット情報

  • 機能
    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • 配列類似性
    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • ドメイン
    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • 細胞内局在
    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker 画像

  • HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.

  • ab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.

    See Abreview

Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (ab12894) 使用論文

This product has been referenced in:
  • Liu Y  et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . Read more (PubMed: 22622241) »
  • Shen Y  et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . Read more (PubMed: 21487022) »

See all 3 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
Paraformaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Apr 30 2012

Thank you for sharing your results through our Abreview system. I am sorry this antibody did not provide the expected results.

I am sorry to hear that you are experiencing difficulties with this product ab12894 in ICC. Often it is possible to...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 May 25 2010

Application
Immunocytochemistry
Sample
African Green Monkey Cultured Cells (kidney)
Specification
kidney
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7)
Specification
MCF-7
Fixative
Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

投稿 Mar 01 2007

Thank you for your enquiry. I am not sure I understand your question, but did notice that the SwissProt database links on the datasheet point to the cation-dependent MPR, not the cation-independent. If this is your question, I am having the databas...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Oct 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録