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Isolated M13 phage coat proteins
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
Our Abpromise guarantee covers the use of ab9225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||1/25 - 1/200. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).|
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