The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
Sorting of M13 infected cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.