製品の概要
- 製品名Anti-M-CSF antibody
M-CSF 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to M-CSF
- アプリケーション適用あり: IHC-P, WBmore details
- 種交差性交差種: Mouse, Human
交差が予測される動物種: Dog, Pig, Macaque monkey, Gorilla
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MCSF.
- ポジティブ･コントロール
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Spleen; Thymus; Tonsil. It also gave a positive result in FFPE human tonsil tissue sections.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab99178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). |
ターゲット情報
- 機能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
- 翻訳後修飾Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. - 細胞内局在Cell membrane and Secreted > extracellular space.
- Information by UniProt
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参照データベース
- Entrez Gene: 1435 Human
- Entrez Gene: 12977 Mouse
- Omim: 120420 Human
- SwissProt: P09603 Human
- SwissProt: P07141 Mouse
- Unigene: 591402 Human
- Unigene: 795 Mouse
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別名
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Anti-M-CSF antibody 画像
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Western blot - Anti-MCSF antibody (ab99178)All lanes : Anti-M-CSF antibody (ab99178) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 170 kDa,52 kDa (possible cleavage fragment),98 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutesMCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCSF antibody (ab99178)IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCSF antibody (ab99178)This image is courtesy of an anonymous Abreviewab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
Anti-M-CSF antibody (ab99178) 使用論文
ab99178 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"