Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MCSF.
Our Abpromise guarantee covers the use of ab99178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). |
ab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
ab99178 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"