製品の概要

  • 製品名Anti-M-CSF antibody
    M-CSF 一次抗体 製品一覧
  • 製品の詳細
    Mouse Monoclonal to M-CSF
  • アプリケーション適用あり: Flow Cyt, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment with tag: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK, corresponding to amino acids 33-132 of Human MCSF

  • ポジティブ・コントロール
    • MCSF transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • 翻訳後修飾Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • 細胞内局在Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Anti-M-CSF antibody 画像

  • All lanes : Anti-M-CSF antibody (ab66236) at 1 µg/ml

    Lane 1 : MCSF transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 60 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HL60 cells stained with ab66236 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66236, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1](ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 4% paraformaldehyde (10 min) and permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-M-CSF antibody (ab66236) 使用論文

This product has been referenced in:
  • Woo HH  et al. Nucleolin mediates microRNA-directed CSF-1 mRNA deadenylation but increases translation of CSF-1 mRNA. Mol Cell Proteomics 12:1661-77 (2013). Read more (PubMed: 23471483) »

See 1 Publication for this product

Product Wall

Thank you very much for your call today and for letting us know about the continued problems with the recent lots of this antibody.

As we discussed, I'm sending a free of chargevial of ab52864 on the order *** which should arrive tomorrow. A ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"