製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LZTR1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: QAYCKQNLEM NVTVQNVLQI LEAADKTQAL DMKRHCLHII VHQFTKVSKL, corresponding to a region within C terminal amino acids 757-806 of Human LZTR1

  • ポジティブ・コントロール
    • Jurkat cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titer using peptide based assay: 1:312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 95 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • All lanes : Anti-LZTR1 antibody (ab66648) at 2.5 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : (A) Molecular Marker
    Lane 2 : (B) Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 95 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab66648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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