製品の概要

  • 製品名Anti-LYVE1 antibody
    LYVE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LYVE1
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Dog, Human
  • 免疫原

    Recombinant fragment corresponding to Human LYVE1 aa 24-232. Recombinant human soluble LYVE1 fragment from insect cells (ab54341).
    (Peptide available as ab54341)

製品の特性

  • 製品の状態Lyophilised:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Recombinant Human LYVE1 protein (ab54341).
IHC-P Use a concentration of 6 - 30 µg/ml.
IHC-Fr Use a concentration of 6 - 30 µg/ml.
Flow Cyt Use a concentration of 3 - 10 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 組織特異性Mainly expressed in endothelial cells lining lymphatic vessels.
  • 配列類似性Contains 1 Link domain.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody 画像

  • IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
  • The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.

  • All lanes : Anti-LYVE1 antibody (ab36993)

    Lane 1 : Recombinant Human LYVE1 protein (ab54341)
    Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)


    Predicted band size : 35 kDa
  • Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.

Anti-LYVE1 antibody (ab36993) 使用論文

This product has been referenced in:
  • Davis JM  et al. Lymphatic endothelial differentiation in pulmonary lymphangioleiomyomatosis cells. J Histochem Cytochem 61:580-90 (2013). Human . Read more (PubMed: 23609227) »
  • Bonneau C  et al. Lymphatic and nerve distribution throughout the parametrium. Gynecol Oncol 131:708-13 (2013). IHC-Fr ; Human . Read more (PubMed: 24125751) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 40 min 100C
Specification breast cancer
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 13 2015


ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0,05%
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Álvaro Teijeira

Verified customer

投稿 Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"