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Our Abpromise guarantee covers the use of ab10278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 6 - 30 µg/ml.
Fix sections for 10 min at -20°C in MeOH.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35-45 kDa (predicted molecular weight: 35 kDa).Can be blocked with Recombinant Human LYVE1 protein (ab54341).|
Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.
Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.