The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Mainly expressed in endothelial cells lining lymphatic vessels.
Contains 1 Link domain.
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
ICC/IF analysis of LYVE-1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LYVE-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).