遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Lysophospholipase 1 抗体 [EPR3667] (ab91606)

製品の概要

  • 製品名
    Anti-Lysophospholipase 1 antibody [EPR3667]
    Lysophospholipase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3667] to Lysophospholipase 1
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide coprresponding to residues in human acyl protein thioesterase 1.

  • ポジティブ・コントロール
    • WB: fetal liver, fetal heart, Human spleen, Mouse heart, Mouse kidney, Mouse spleen and Rat kidney lysates IHC: Human brain
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR3667
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Predicted molecular weight: 25 kDa.
IP 1/10.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    画像



    • Predicted band size : 25 kDa

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
      Lanes 1 - 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91606 was shown to specifically react with LYPLA1 when LYPLA1 knockout samples were used. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE.  ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Lysophospholipase 1 antibody [EPR3667] (ab91606) at 1/5000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : Fetal heart lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 25 kDa
    • All lanes : Anti-Lysophospholipase 1 antibody [EPR3667] (ab91606) at 1/5000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 25 kDa
    • ab91606, at a 1/100 dilution, staining Lysophospholipase 1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Gardner JK  et al. Lipid-laden partially-activated plasmacytoid and CD4(-)CD8a(+) dendritic cells accumulate in tissues in elderly mice. Immun Ageing 11:11 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25089147) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (muscle)
    Loading amount
    20 µg
    Specification
    muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録