製品の概要

  • 製品名Anti-LYRIC antibody [EP4445]
    LYRIC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4445] to LYRIC
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human LYRIC.

  • ポジティブ・コントロール
    • HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/500.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
    • 組織特異性Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
    • 細胞内局在Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3D3 antibody
      • 3D3/LYRIC antibody
      • AEG 1 antibody
      • AEG-1 antibody
      • AEG1 antibody
      • Astrocyte elevated gene 1 antibody
      • Astrocyte elevated gene-1 protein antibody
      • LYRIC antibody
      • LYRIC/3D3 antibody
      • LYRIC_HUMAN antibody
      • Lysine rich CEACAM1 associated protein antibody
      • Lysine rich CEACAM1 co isolated protein antibody
      • Lysine-rich CEACAM1 co-isolated protein antibody
      • Metadherin antibody
      • Metastasis adhesion protein antibody
      • MTDH antibody
      • Protein LYRIC antibody
      see all

    Anti-LYRIC antibody [EP4445] 画像

    • All lanes : Anti-LYRIC antibody [EP4445] (ab124789) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 64 kDa
    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

    • ab124789 at 1/100 dilution staining LYRIC in paraffin-embedded Human brain tissues by Immunohistochemistry.
    • ab124789 at 1/100 dilution staining LYRIC in paraffin-embedded Human breast tissues by Immunohistochemistry.

    Anti-LYRIC antibody [EP4445] (ab124789) 使用論文

    ab124789 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124789.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"