製品の概要

  • 製品名Anti-LYRIC antibody
    LYRIC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LYRIC
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human LYRIC.

    (Peptide available as ab45337.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse and rat skeletal muscle tissue lysates, and in mouse heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skin cancer.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab45338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).Can be blocked with Human LYRIC peptide (ab45337).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
  • 組織特異性Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
  • 細胞内局在Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3D3 antibody
    • 3D3/LYRIC antibody
    • AEG 1 antibody
    • AEG-1 antibody
    • AEG1 antibody
    • Astrocyte elevated gene 1 antibody
    • Astrocyte elevated gene-1 protein antibody
    • LYRIC antibody
    • LYRIC/3D3 antibody
    • LYRIC_HUMAN antibody
    • Lysine rich CEACAM1 associated protein antibody
    • Lysine rich CEACAM1 co isolated protein antibody
    • Lysine-rich CEACAM1 co-isolated protein antibody
    • Metadherin antibody
    • Metastasis adhesion protein antibody
    • MTDH antibody
    • Protein LYRIC antibody
    see all

Anti-LYRIC antibody 画像

  • All lanes : Anti-LYRIC antibody (ab45338) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : DU145 (Human Prostate carcinoma epithelial like cell line) Whole Cell lysate
    Lane 5 : PC3 whole cell lysate (ab3954)
    Lane 6 : U87 MG nuclear extract lysate (ab14903)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,80 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.
  • All lanes : Anti-LYRIC antibody (ab45338) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa


    Exposure time : 1 minute
  • IHC image of LYRIC staining in Human skin cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45338, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • ICC/IF image of ab45338 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45338, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells fixed in 100% methanol (10 min) cells.

Anti-LYRIC antibody (ab45338) 使用論文

This product has been referenced in:

See all 2 Publications for this product

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Expiration date: 22-02-2013
Value: £234

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The concentration of this product is lot dependant. Current lot is at conc.1mg/ml.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (meiotic egg extract)
Specification meiotic egg extract
Fixative pre-fixation in formaldehyde and Methanol fixation after extract spindown onto coverslips
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 03 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 HS578T PC3)
Loading amount 20 µg
Specification MCF7 HS578T PC3
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Dec 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"