製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to LYPLAL1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    DRFKITNDCPEHLE

    , corresponding to internal sequence amino acids 79-92 of Human LYPLAL1 (NP_620149.1)

  • ポジティブ・コントロール
    • Human Adipose and Heart lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab77640 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-Fr
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • Anti-LYPLAL1 antibody (ab77640) at 0.3 µg/ml + Human adipose lysate in RIPA buffer at 35 µg

      Predicted band size : 26 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence analysis of mouse retina, retinal pigment epithelium and sclera in induced myopic eye (bottom left), fellow non-occluded eye (bottom right), and independent control eye (top right).

      LYPLAL1 (green) was stained using ab77640 at 1/200 dilution. A fluorescein-conjugated rabbit anti-goat secondary antibody was used at 1/800 dilution. Nuclei were stained with DAPI (blue).

      Negative control eye (top left) was treated with the secondary antibody alone and DAPI.

    参考文献

    This product has been referenced in:
    • Fan Q  et al. Genetic variants on chromosome 1q41 influence ocular axial length and high myopia. PLoS Genet 8:e1002753 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22685421) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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